| WNT3

Enables protein domain specific binding activity. Involved in positive regulation of Wnt signaling pathway. Acts upstream of or within several processes, including axis specification; embryonic morphogenesis; and regulation of extent of cell growth. Located in extracellular matrix. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; embryo ectoderm; and sensory organ. Human ortholog(s) of this gene implicated in tetraamelia syndrome 1. Orthologous to human WNT3 (Wnt family member 3).
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.38	1.47	0.84	0.48	0.32	0.25	0.2	0.09
Corrected OD	2.29	1.38	0.75	0.39	0.23	0.16	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse WNT3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	94
Plasma(n=8)	92-106	103

 

| Linearity

Samples were spiked with high concentrations of Mouse WNT3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-96	88-95
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	98